BL21 (DE3) RIL competent cells to express tau protein Article

BL21 (DE3) RIL competent cells to express tau protein - Article Example The BL21-CodonPlus(DE3)-RP cells possess excess of the argU which code identifies the arginine codons AGA, tRNAs which encodes the proline codon CCC and proL genes which code tRNAs which identifies the arginine codons AGG. The BL21-CodonPlus (DE3)-RIPL cells contain extra copies of the argU, ileY, and leuW as well as the positive tRNA genes (Sussman, & Israel 218). This particular strain liberates the appearance of heterologous proteins obtainable from organisms that possess each AT- or GC containing genomes (Sussman, & Israel 220). BL21 (DE3) RIL is important in the expression of the tau protein since it promotes the optimal protein transformation. The Tau proteins are steadies microtubules, they are copious in nerves in the CNS and are least frequent somewhere else (Robbins & Maria 230). BL21 (DE3) RIL is important in the assembly of tubulin assisting in the optimal transformation of cells that induce the expression of tau protein. Tau possesses two N-terminal exons and has all the microtubule attaching repeats in the largest human tau isoform. This promotes the transformation and the expression of the tau protein in the medium (Robbins & Maria

Art history Essay Example | Topics and Well Written Essays - 500 words - 2

Art history - Essay Example The painting clearly shows the image of a young mother nursing a child and a man standing with a spear. On the background there is a river passing by and visible buildings on the river bank. The man is guarding the woman who has probably just given birth. Poesia describes the works of Italian Renaissance that were inspired by myths and fables. The painting has an elaborate use of light and color which is a hallmark characteristic of Venetian paintings. The author of the work also used canvas. The use of mannerisms to depict human beings in distorted poses is also evident of Venetian painting (Humfrey, 132). Meeting of Bacchus and Ariadne depicts the magnificence of art through the images of strong men and women in some small setting. On the other hand Botticelli’s Birth of Venus shows the sensual evocation as depicted by the impressive looking Goddess of love in Venus. An Apocalypse refers to a revelation of something with hidden meaning. In the Christian context, Apocalypse generally refers to the revelation of some things which are hidden from human beings. The second figure from the fore represents death as it is bigger and scarier than the rest. The painting is woodcut the relief images are cut onto the surface of the wood. DÃ ¼rer depicts himself in the self portrait as a God. The implied position of an artist is that of divine nature akin to that of God to create. An artist’s role in the society had grown in stature. This is because artists were able to create image that were subject to various interpretations by the audience. DÃ ¼rer painted this picture to portray the present conditions in terms of religion during his time. It should be noted that the title of the painting is misleading as there are actually three apostles in the painting. The painting was presented to the magistrates after the Reformation. The painting seems to appreciate the role of scripture in the society but warns against false teachings. In essence,

Interview Reflection Worksheet Coursework Example | Topics and Well Written Essays - 250 words

Interview Reflection Worksheet - Coursework Example My weak point is in competence that majorly rises from my inability to make some decisions under pressure effectively. Competence will improve when I gain the necessary work experience and gain more knowledge in my area of operation. The assessment of people to some extent showed me the various points of weakness. I realised that I need to improve on competence and efficiency in decision-making. The alignment of people’s perception and mine shown that i am ethical similar to my personal assessment. My surprise was the knowledge of other people that i am incompetent due to the minimal experience I possess. It was not easy to stay open minded and non-defensive during the interview. The questions were touching on my personality and i felt like faking and lying about some aspects of the issues under discussion. I achieved this by trying to be ethical and comprehensively cooperative. I am being perceived this way because am truthful to my acts. My partner was ethical and tried to corner my answers but due to professionalism and adhering to the set standards, he got the right information that provided the right illustration of my characters. However, the results were not as per my expectations but they are a true reflection of my

In what ways are horses used as symbolism in the plays The New York Essay

In what ways are horses used as symbolism in the plays The New York Idea by Landgon Mitchell, and the play Mazeppa by Henry - Essay Example In order to understand why horses are used in both stories, we must take a closer look at the general perception of what horses stand for. According to Psychics and Mediums Network, a fantastic website dedicated to the interpretation of dreams and symbols, horses in a dream mean the following: Wild forces that have been tamed are symbolized by the horse. Horses are also a symbol of sexuality and were considered by Freud to represent the terrifying aspect of the father. To dream of ridding an out-of-control horse, may indicate that you are being ‘carried away’ by your passions, whereas a tightly tethered horse may show that you inhibit these natural feelings. Therefore, horses reflect on the feelings of individuals—where they are going and what they are doing. This concept is certainly used in both Mitchell and Milner’s works. As noted above, horses are used to judge different individual actions. In the introduction of â€Å"The New York Idea,† Mitche ll states, interestingly enough: â€Å"The temperament of the race-horse has to be considered—much more, that of the 'star'.† (2). This is an important concept in relation to what we have already been discussing. The author is basically admitting the purpose of the horses in the play itself—to reflect on the actions and emotions of individuals. In Mazeppa, we see a similar stance. The bulk of the narrative focuses on a romantic journey on a horse, the horse serving as sort of escape from reality, or one’s emotions and personal responsibilities. This is especially accurate if we compare this with the Freudian interpretation of horses above. Horses can represent one being carried away by one’s passions, and this seems to be in line with what is going on in Mazeppa. Therefore, the general interpretation of what horses can mean symbolically certainly ties back to what Mazeppa is trying to reveal a horse can mean. Another concept present in Psychics and Mediums network is the reflection of what horses generally mean in mythological terms, and again, this concept can be used to better understand the symbols present in both stories that we are discussing. According to Psychics and Mediums Network, horses can myth logically mean the following: In some myths and fairytales horses speak. In dreams, this represents the voice of your unconscious- a message from your innermost self. In Greek myth, horses were associated with Hades, the underworld and death. However, dream prophecy says that to dream of horses indicates that you will receive news from a distance. And it is extremely lucky to dream of a horse being shod. So, the journey of the horse in â€Å"Mazeppa† can be related to the ultimate end of all human journeys—that of death. Basically, there is the sense that no matter what we do with our lives, all of us will eventually face this end of the journey, or the ultimate end, of relinquishing our souls. This concept is not as present in The New York Idea, but if we think of the races present in the story as a type of journey for the horses, then we see a similar concept present; that of the journey and the finish, and whether ones wins or loses it does not really seem to matter when one reaches the final end of all things. The other interesting point to note about this interpretation of horses is the concept of receiving news from a dista

Interview Reflection Worksheet Coursework Example | Topics and Well Written Essays - 250 words

Interview Reflection Worksheet - Coursework Example My weak point is in competence that majorly rises from my inability to make some decisions under pressure effectively. Competence will improve when I gain the necessary work experience and gain more knowledge in my area of operation. The assessment of people to some extent showed me the various points of weakness. I realised that I need to improve on competence and efficiency in decision-making. The alignment of people’s perception and mine shown that i am ethical similar to my personal assessment. My surprise was the knowledge of other people that i am incompetent due to the minimal experience I possess. It was not easy to stay open minded and non-defensive during the interview. The questions were touching on my personality and i felt like faking and lying about some aspects of the issues under discussion. I achieved this by trying to be ethical and comprehensively cooperative. I am being perceived this way because am truthful to my acts. My partner was ethical and tried to corner my answers but due to professionalism and adhering to the set standards, he got the right information that provided the right illustration of my characters. However, the results were not as per my expectations but they are a true reflection of my

LEGO Case Study - Strategic Management Essay Example | Topics and Well Written Essays - 1500 words

LEGO Case Study - Strategic Management - Essay Example In the 80-year history of the company, the last fifteen years or so have been one of happenings periods in the history of company. From its peak in the mid 90s to a serious slump in the late 1990s and early 2000s and then from that to a period of recovery and dominance, the period of 1995-2009 has many stories to tell for Lego (Ireland, Hoskisson & Hitt, pp. 85-89, 2008). Problems for the company started somewhere in the 1990s but they did not made the headlines or even got the attention of the top management of the company till 1998 when the company incurred its first loss on its income statement in its entire history. Lego got back on the track of making profits during 2001 and 2002 but that was primarily because of the deals with Harry Potter franchise and when no Harry Potter movie was released during 2003, the company’s sales decreased by 26 percent and 20 percent in the subsequent year (The Lego Group, 2012). Furthermore, the company posted a record loss of over 240 mill ion US dollars and rumors started in the market that Mattel would soon takeover Lego (Lewis, pp. 21-23, 2011). There were many reasons behind the declining market share of the company. First, the toy industry was going through a similar revolution and competitive outburst as of the airline industry. Many competitors with low cost and claiming to be high quality, entered into the market to disrupt the equilibrium. For example, a Canadian toy company, Megabloks was instrumental in removing Lego toys from many shelves because not only it was of lower price to attract the customers but also even the retailers were paid with higher profit margins. Second, many of the customers of the company that were young boys were being more attracted to computer games and video games during that time thus decreasing the sales even further. Third, Lego’s main operations were in Denmark, which was high cost economy with long supply chains and many supplies. In fact, it at one point in time, Lego had more suppliers than Boeing had to build aircrafts (Ireland, Hoskisson & Hitt, pp. 85-89, 2008). Strategies In order to understand the strategies used by Lego during the period of 1995-2009, we will have to divide the period between two sub-periods of 1998-2003 and 2004-2009 since the company shifted its strategy during the period. First period (1998-2003) During the first period of 1998-2003 and even before that, Lego was following an aggressive policy of product development. Lego was constantly trying to improve its existing products with bringing new variations. The mindset of the company’s strategists was that by providing more product variations, they would force the customers to spend more and new variations would bring more and new customers into the loop (Bender, pp. 55, 2010). In fact, it appeared that somehow Lego was trapped in the product orientation, one of the five marketing orientations. In it, the prime focus and expectation of the company was on improving the current products, bringing more colour, designs and variations hoping that would make the customers stay and even become ready to pay a premium for Lego’s products (Ireland, Hoskisson & Hitt, pp. 85-89, 2008). Secondly, this was a period where Lego engaged in many strategic partners with Harry Potter, Disney and other franchises in an attempt to co-brand their products. In fact, this was the prime reason why Lego was able to show some profit during these years (The Lego Group, 2012). Second

Management Philosophy Essay Example for Free

Management Philosophy Essay Diversity trainer through the National Multi-Cultural Institute (NMCI) which is based out of Washington DC. Bahaudin worked as a manager, an Internal Consultant, Trainer, and Teacher at the Education and Training Development Department of Human Resources with Publix Super Markets Inc. for sixteen years. Bahaudin has been a visitor or speaker on conferences in the United States of America, Vietnam, Malaysia, Afghanistan, Pakistan, India, Brazil, Jamaica, Bahamas, St. Lucia, Thailand, Myanmar (Burma), Grenada, and several other Caribbean countries. Bahaudin was born in Khoshie of Logar and raised in Kabul of Afghanistan. Bahaudin finished his high school degree and higher education in the United States. Management Philosophy: Some of Bahaudin’s favorite management concepts, which he has used in practice, happen to be Self-fulfilling Prophecy, Theory Y View of Motivation, Management by Objective and Management by Walking Around. Managers are likely to get exactly what they expect from themselves and their employees. Bahaudin believes that most people want to do a good job, especially when they are given the right tools, educational developments and performance opportunities. He prefers leading people and managing systems. Bahaudin likes to clarify his overall objectives, set realistic goals and then work to achieve them in a realistic timeframe. According to Bahaudin, the journey of working toward the achievement of one’s goals itself can certainly be one way to happiness. As they say, happiness is the way. Bahaudin truly believes that happiness is a journey, and not a destination. Happiness is the progressive realization of worthwhile and predetermined goals. So, set your goals and, as someone said, then â€Å"work like you dont need money; study like you are a noble prize winner; love like youve never been hurt; and dance like no ones watching. Have a positive attitude and, when possible, make a difference in at least one person’s life. Remember, if you can perceive and believe a better state of being, then you are very likely to achieve it as well. Overall, learn as much as you can; stretch yourself as far as possible, but not beyond; never settle for less than your capabilities; aim for total integrity; and be the best that you can be! As an effective manager and leader, may you have the hindsight to know where you have been; the foresight to know where you are going; and the insight to know when you are about to go too far.